GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM325437 Query DataSets for GSM325437
Status Public on Feb 01, 2010
Title TS 3.5 day 1
Sample type RNA
Source name TS cells differentiated day 1
Organism Mus musculus
Characteristics 129S6 genetic background
Treatment protocol To differentiate TS cell FGF4 heparin and feeder conditioned medium were all withdrawn from the media.
Growth protocol TS cells were grown feeder free with 30% feeder conditioned medium, RPMI, FGF4 and heparin as per Tanaka et al , 1998 PMID:9851926
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A second clean up step was done using the RNEasy kit from QIAGEN according to manufactures instructions
Label biotin
Label protocol as per facility protocols
Hybridization protocol as per facility protocols
Scan protocol Gene Chips were scanned using the Hewlett-Packard Gene Array Scanner G2500A.
Description gene expression data of TS cells differentiated day 1
Data processing The data were analyzed with Expression Console (Affymetrix) using the quartile normalization and PLIER algorithm
Submission date Sep 30, 2008
Last update date Nov 23, 2009
Contact name Brian Joseph Cox
Organization name University of Toronto
Department Physiology
Lab Cox System Biology
Street address 1 King's College Circle, Rm 3360
City Toronto
State/province Ontario
ZIP/Postal code M5S1A8
Country Canada
Platform ID GPL1261
Series (2)
GSE12985 Differentiation time course of trophoblast stem cells
GSE12999 Gata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development

Data table header descriptions
VALUE Quantification

Data table
AFFX-BioB-5_at 765.47
AFFX-BioB-M_at 876.29
AFFX-BioB-3_at 638.11
AFFX-BioC-5_at 1256.42
AFFX-BioC-3_at 1242.43
AFFX-BioDn-5_at 2256.95
AFFX-BioDn-3_at 5094.52
AFFX-CreX-5_at 12379.1
AFFX-CreX-3_at 13792.6
AFFX-DapX-5_at 32.7618
AFFX-DapX-M_at 39.8646
AFFX-DapX-3_at 8.76128
AFFX-LysX-5_at 16.4526
AFFX-LysX-M_at 38.4338
AFFX-LysX-3_at 31.8592
AFFX-PheX-5_at 26.4022
AFFX-PheX-M_at 128.459
AFFX-PheX-3_at 95.1089
AFFX-ThrX-5_at 30.594
AFFX-ThrX-M_at 168.286

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.

Supplementary file Size Download File type/resource
GSM325437.CEL.gz 4.1 Mb (ftp)(http) CEL
GSM325437.chp.gz 323.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap