|
| Status |
Public on Jul 06, 2018 |
| Title |
WT rep2 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Yorkshire
genotype/variation: WT
age: 6 months
cell type: PBMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the standard TRIzol protocol.
Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
F1_5
processed data file: processed_data.txt
|
| Data processing |
The raw data was checked by FastQC v.
Clean reads were aligned to the pig genome using STAR 2.5.
The expression of the transcript was calculated by FPKM using Cuffnorm.
Differentially expressed transcripts were determined using DESeq v1.22.1.
Function and signaling pathway enrichment analysis using GO and KEGG database.
Genome_build: Sscrofa 10.2
Supplementary_files_format_and_content: processed_data.txt: Tab-delimited text file includes FPKM values for all Samples.
|
| |
|
| Submission date |
Jul 05, 2018 |
| Last update date |
Jul 08, 2018 |
| Contact name |
junchen chen |
| E-mail(s) |
499975690@qq.com
|
| Phone |
8613907488785
|
| Organization name |
1Department of Dermatology, Xiangya Hospital, Central South University
|
| Department |
1Department of Dermatology, Xiangya Hospital, Central South University
|
| Street address |
87 Xiangya Road, Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China.
|
| City |
changsha |
| ZIP/Postal code |
400001 |
| Country |
China |
| |
|
| Platform ID |
GPL22918 |
| Series (1) |
| GSE116701 |
Analysis of Wild Type and Leptin-overexpressing Pig PBMC Transcriptomes |
|
| Relations |
| BioSample |
SAMN09621208 |
| SRA |
SRX4345477 |
