|
| Status |
Public on Sep 28, 2009 |
| Title |
Bexp_Kstat_dyeswap2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MG1655, stationary phase, LB
|
| Organism |
Escherichia coli |
| Characteristics |
MG1655, stationary phase, LB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells grown in LB medium using the Qiagen RNeasy column.
|
| Label |
Cy3
|
| Label protocol |
Fluorescently-labeled cDNA was synthesized by reverse transcription of total RNA with aminoallyl-dUTP and random primers followed by the coupling of Cy3 or Cy5 dyes (Amersham Pharmacia).
|
| |
|
| Channel 2 |
| Source name |
REL606, exponential phase, LB
|
| Organism |
Escherichia coli |
| Characteristics |
REL606, exponential phase, LB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells grown in LB medium using the Qiagen RNeasy column.
|
| Label |
Cy5
|
| Label protocol |
Fluorescently-labeled cDNA was synthesized by reverse transcription of total RNA with aminoallyl-dUTP and random primers followed by the coupling of Cy3 or Cy5 dyes (Amersham Pharmacia).
|
| |
|
| |
| Hybridization protocol |
Hybridization and washes were performed according to the instruction of the manufacturer (Digital Genomics).
|
| Scan protocol |
The hybridized slides were scanned with GenePix 4000B (Molecular Devices) and scanned images were analyzed by GenePix Pro 6.0.
|
| Description |
The microarray contains two large blocks, onto each of which the probes were spotted in duplicate. The data is for one of the blocks (dye-swap experement).
|
| Data processing |
Following background subtraction, spot signal intensities were calculated as percentages of total signal intensities of Cy-3 or Cy-5 signals on each microarray, as a means of normalization. Spots with low intensities were excluded.
|
| |
|
| Submission date |
Oct 01, 2008 |
| Last update date |
Oct 03, 2008 |
| Contact name |
Sung Ho Yoon |
| Organization name |
Konkuk University
|
| Department |
Department of Bioscience and Biotechnology
|
| Street address |
120 Neungdong-ro, Gwangjin-gu
|
| City |
Seoul |
| ZIP/Postal code |
05029 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL7395 |
| Series (1) |
| GSE13011 |
Transcriptomic differences between E. coli B REL606 and K-12 MG1655 |
|
