|
| Status |
Public on Apr 29, 2019 |
| Title |
Cuz_31 |
| Sample type |
SRA |
| |
|
| Source name |
mice expressing HA-tagged ribosomal protein RPL22 in oligodendrocytes, Cuz
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Corpus callosum
cell type: oligodendrocytes
treatment: fed with 0.2% bis-(cyclohexanone)-oxaldihydrazone (cuprizone) diet
rip antibody: monoclonal anti-HA antibody
|
| Treatment protocol |
Young adult female mice (age 8-10 weeks) were fed with 0.2% bis-(cyclohexanone)-oxaldihydrazone (cuprizone) diet (TD.140803, Harlan Teklad) for 6 weeks or 9 weeks to induce demyelination, followed by a 3 week recovery period on global 18% protein rodent control diet (TD.00588, Harlan Teklad) to induce natural remyelination.
|
| Growth protocol |
Animals were maintained under standard conditions in a 12 h dark/12 h light cycle with access to food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Corpus callosum was collected and snap-frozen by liquid nitrogen. After RNA co-immunoprecipitation process, RNA was isolated using Direct-zol™ RNA MiniPrep Plus kit (Zymo Research).
Double-stranded cDNA and the sequencing library were made using the Kapa RNA Kit Full kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Data quality check was done on Illumina SAV
De-multiplexing was performed with Illumina Bcl2fastq2 v 2.17 program
R package “QuasR” was used for the read alignment to mouse genome (mm10) followed by the counting at gene level
Genome_build: mm10
Supplementary_files_format_and_content: "Olig1.RiboTag_rawcounts.txt" has count values for each Sample
|
| |
|
| Submission date |
Aug 13, 2018 |
| Last update date |
Apr 29, 2019 |
| Contact name |
Yuichiro Itoh |
| E-mail(s) |
yitoh@ucla.edu
|
| Phone |
3102068999
|
| Organization name |
UCLA
|
| Department |
Neurology
|
| Street address |
635 Charles E. Young Drive South
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE118451 |
Gene expression in oligodendrocytes during remyelination reveals cholesterol homeostasis as a therapeutic target in multiple sclerosis. |
|
| Relations |
| BioSample |
SAMN09812713 |
| SRA |
SRX4548293 |
