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Status |
Public on Aug 15, 2018 |
Title |
Identificaton of abberantly expressed genes in pooled RNA sample from 15 breast cancer specimens |
Sample type |
RNA |
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|
Source name |
15 breast cancer specimens
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast cancer
|
Growth protocol |
Normal cell culture medium RPMI 1640 or DMEM.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol following the manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
|
|
Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution. Images were quantified using Agilent Feature Extraction software version 10.7.3.1
|
Data processing |
LOWESS normalized, background subtracted VALUE data obtained from Feature Extraction. Agilent software was used.
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Submission date |
Aug 14, 2018 |
Last update date |
Aug 15, 2018 |
Contact name |
Naohiko Seki |
E-mail(s) |
naoseki@faculty.chiba-u.jp
|
Phone |
+81-43-226-2971
|
Organization name |
Chiba University
|
Department |
Functional Genomics
|
Street address |
1-8-1, Inohana
|
City |
Chiba |
State/province |
Chiba |
ZIP/Postal code |
260-8670 |
Country |
Japan |
|
|
Platform ID |
GPL20844 |
Series (1) |
GSE118539 |
Differentially expressed genes in human brest cancer specimens |
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