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| Status |
Public on Jan 11, 2013 |
| Title |
definitive_Alzheimer_4_hippocampus_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
RNA pool of 15 different cell lines
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Protocol Cy3/Cy5
|
| |
|
| Channel 2 |
| Source name |
dAD4H
|
| Organism |
Homo sapiens |
| Characteristics |
braak: 5 CERAD: A CDR: 3 Gender: female Age: 83 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit Pico Pure RNA Isolation Kit
|
| Label |
Cy5
|
| Label protocol |
Protocol Cy3/Cy5
|
| |
|
| |
| Hybridization protocol |
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Washing - Round 1 - Solution: SSC 2x / Temperature:42 / Time:10 min. / Washing Number: 1
Washing - Round 2 - Solution: SSC 0,1x; SDS 0,1% / Temperature:42 / Time:10 min. / Washing Number: 2
Washing - Round 3 - Solution: SSC 0,1x / Temperature:42 / Time:10 min. / Washing Number: 2
|
| Scan protocol |
Hardware: Perkinelmer / Software: ScanArray
|
| Description |
Reference RNA: Daudi (Burkitt's lymphoma), DLD-1 (colorectal adenocarcinoma), DU 145 (prostate carcinoma), FaDu (pharinx squamous cell carcinoma), GM-637 (fibroblast), H-146 (small cell lung carcinoma), HT-1080 (fibrosarcoma), HB4a (normal mammary luminal epithelium), HEK (human embryonic kidney), Jurkat (acute T cell leukemia), Saos-2 (osteosarcoma), SK-BR-3 (breast adenocarcinoma), SK-MEL-28 (melanoma), T24 (bladder transitional cell carcinoma), T98G (glioblastoma multiforme).
|
| Data processing |
Lowesss
|
| |
|
| Submission date |
Oct 15, 2008 |
| Last update date |
Jun 12, 2013 |
| Contact name |
Renato David Puga |
| Organization name |
Universidade de São Paulo
|
| Department |
Instituto de Psiquiatria
|
| Lab |
Laboratório de Genética
|
| Street address |
Dr Ovidio Pires de Campos,785
|
| City |
São Paulo |
| State/province |
São Paulo |
| ZIP/Postal code |
05403-010 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL1930 |
| Series (1) |
| GSE13214 |
Evaluation of gene expression profile in postmortem brain with Alzheimer´s disease-type neuropathological changes |
|
| Data table header descriptions |
| ID_REF |
this column should correspond to the ID column of the reference platform |
| IntCH1 |
Channel 1 Intensity |
| BkgCH1 |
BackGround Channel 1 |
| IntCH2 |
Channel 2 Intensity |
| BkgCH2 |
BackGround Channel 2 |
| VALUE |
-[INV_VALUE]: Lowess-normalized log2(Cy3/Cy5) ratios |
| INV_VALUE |
Lowess-normalized log2(Cy3/Cy5) ratios |
| Data table |
| ID_REF |
IntCH1 |
BkgCH1 |
IntCH2 |
BkgCH2 |
VALUE |
INV_VALUE |
| 1 |
13916 |
156 |
4660 |
84 |
null |
null |
| 2 |
351 |
109 |
91 |
87 |
null |
null |
| 3 |
4794 |
149 |
5411 |
93 |
-1.43169 |
1.43168578992696 |
| 4 |
798 |
144 |
116 |
88 |
-0.834623 |
0.834622598370331 |
| 5 |
1499 |
135 |
209 |
88 |
-1.04382 |
1.04382285460697 |
| 6 |
3154 |
134 |
116 |
90 |
2.23359 |
-2.23358885687080 |
| 7 |
568 |
132 |
107 |
86 |
-1.22322 |
1.22322356563095 |
| 8 |
1056 |
143 |
482 |
91 |
-3.48095 |
3.48095497031628 |
| 9 |
925 |
143 |
95 |
88 |
1.05711 |
-1.05711110104902 |
| 10 |
2170 |
146 |
121 |
86 |
1.19799 |
-1.19798757477168 |
| 11 |
297 |
103 |
86 |
85 |
null |
null |
| 12 |
315 |
110 |
88 |
87 |
null |
null |
| 13 |
520 |
119 |
114 |
91 |
-1.47264 |
1.47263930816506 |
| 14 |
793 |
138 |
99 |
88 |
0.219997 |
-0.219997355496028 |
| 15 |
28442 |
151 |
386 |
87 |
4.05113 |
-4.05113035423282 |
| 16 |
448 |
130 |
95 |
89 |
-0.514969 |
0.514968674268987 |
| 17 |
523 |
128 |
109 |
89 |
-1.3464 |
1.34640132241889 |
| 18 |
7736 |
142 |
116 |
87 |
3.44554 |
-3.4455405880165 |
| 19 |
386 |
119 |
92 |
84 |
-1.10567 |
1.1056653471034 |
| 20 |
5310 |
149 |
109 |
89 |
3.43987 |
-3.43986514946539 |
Total number of rows: 4800
Table truncated, full table size 216 Kbytes.
| Supplementary data files not provided |
| Processed data included within Sample table |
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