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Status |
Public on Jan 11, 2013 |
Title |
pathologic_Alzheimer_4_hippocampus_rep2 |
Sample type |
RNA |
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Channel 1 |
Source name |
Reference RNA
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Organism |
Homo sapiens |
Characteristics |
RNA pool of 15 different cell lines
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Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
Protocol Cy3/Cy5
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Channel 2 |
Source name |
pAD4H
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Organism |
Homo sapiens |
Characteristics |
braak: 6 CERAD: C CDR: 3 Gender: female Age: 86 years
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Extracted molecule |
total RNA |
Extraction protocol |
kit Pico Pure RNA Isolation Kit
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Label |
Cy5
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Label protocol |
Protocol Cy3/Cy5
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Hybridization protocol |
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down: Washing - Round 1 - Solution: SSC 2x / Temperature:42 / Time:10 min. / Washing Number: 1 Washing - Round 2 - Solution: SSC 0,1x; SDS 0,1% / Temperature:42 / Time:10 min. / Washing Number: 2 Washing - Round 3 - Solution: SSC 0,1x / Temperature:42 / Time:10 min. / Washing Number: 2
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Scan protocol |
Hardware: Perkinelmer / Software: ScanArray
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Description |
Reference RNA: Daudi (Burkitt's lymphoma), DLD-1 (colorectal adenocarcinoma), DU 145 (prostate carcinoma), FaDu (pharinx squamous cell carcinoma), GM-637 (fibroblast), H-146 (small cell lung carcinoma), HT-1080 (fibrosarcoma), HB4a (normal mammary luminal epithelium), HEK (human embryonic kidney), Jurkat (acute T cell leukemia), Saos-2 (osteosarcoma), SK-BR-3 (breast adenocarcinoma), SK-MEL-28 (melanoma), T24 (bladder transitional cell carcinoma), T98G (glioblastoma multiforme).
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Data processing |
Lowesss
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Submission date |
Oct 15, 2008 |
Last update date |
Jun 12, 2013 |
Contact name |
Renato David Puga |
Organization name |
Universidade de São Paulo
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Department |
Instituto de Psiquiatria
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Lab |
Laboratório de Genética
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Street address |
Dr Ovidio Pires de Campos,785
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City |
São Paulo |
State/province |
São Paulo |
ZIP/Postal code |
05403-010 |
Country |
Brazil |
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Platform ID |
GPL1930 |
Series (1) |
GSE13214 |
Evaluation of gene expression profile in postmortem brain with Alzheimer´s disease-type neuropathological changes |
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Data table header descriptions |
ID_REF |
this column should correspond to the ID column of the reference platform |
IntCH1 |
Channel 1 Intensity |
BkgCH1 |
BackGround Channel 1 |
IntCH2 |
Channel 2 Intensity |
BkgCH2 |
BackGround Channel 2 |
VALUE |
-[INV_VALUE]: Lowess-normalized log2(Cy3/Cy5) ratios |
INV_VALUE |
Lowess-normalized log2(Cy3/Cy5) ratios |
Data table |
ID_REF |
IntCH1 |
BkgCH1 |
IntCH2 |
BkgCH2 |
VALUE |
INV_VALUE |
1 |
16714 |
139 |
5552 |
85 |
null |
null |
2 |
347 |
109 |
84 |
84 |
null |
null |
3 |
5608 |
141 |
2460 |
85 |
-1.58655 |
1.58654741629561 |
4 |
587 |
123 |
103 |
85 |
-0.166107 |
0.166107045222905 |
5 |
1582 |
137 |
284 |
83 |
-0.999027 |
0.99902688842591 |
6 |
2002 |
125 |
141 |
84 |
1.12528 |
-1.12527692238409 |
7 |
660 |
117 |
90 |
85 |
1.14377 |
-1.1437727768889 |
8 |
862 |
121 |
272 |
83 |
-1.9237 |
1.92370034819052 |
9 |
918 |
120 |
97 |
83 |
1.13492 |
-1.13492099203107 |
10 |
1274 |
126 |
102 |
83 |
1.54277 |
-1.54276879447372 |
11 |
330 |
110 |
82 |
81 |
null |
null |
12 |
392 |
109 |
86 |
83 |
null |
null |
13 |
1370 |
125 |
282 |
86 |
null |
null |
14 |
683 |
131 |
98 |
85 |
0.467777 |
-0.467776960811539 |
15 |
26716 |
149 |
2125 |
82 |
1.85491 |
-1.85490896758254 |
16 |
442 |
119 |
90 |
84 |
-0.142069 |
0.142068571874829 |
17 |
543 |
118 |
131 |
83 |
-1.26621 |
1.26621192161098 |
18 |
5736 |
145 |
233 |
85 |
1.55417 |
-1.55417074991338 |
19 |
397 |
119 |
90 |
85 |
-0.374691 |
0.3746910951255 |
20 |
5990 |
130 |
258 |
85 |
1.44882 |
-1.44882454022355 |
Total number of rows: 4800
Table truncated, full table size 218 Kbytes.
Supplementary data files not provided |
Processed data included within Sample table |
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