|
| Status |
Public on Aug 07, 2019 |
| Title |
IL2-1-T12 |
| Sample type |
SRA |
| |
|
| Source name |
B16F10 tumors_ACT+IL2
|
| Organism |
Mus musculus |
| Characteristics |
host strain: C57/BL6
implanted with: B16F10 syngeneic murine melanoma cell line
tissue: B16F10 tumors
treatment: adoptive cell transfer (ACT) of pmel-1 T cells with IL-2
time point: day 12 after treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit from Qiagen
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
IL1T12_S19
|
| Data processing |
Reads were mapped using HISAT2 version 2.0.4 and aligned to the Mus musculus genome NCBI build GRCm38.
Reads were quantified HTSeq version 0.6.12 and normalized to fragments per kilobase of exon per million fragments mapped (FPKM) expression values.
Genome_build: Mus musculus genome NCBI build GRCm38
Supplementary_files_format_and_content: Matrix of gene expression measurements in FPKM
|
| |
|
| Submission date |
Aug 19, 2018 |
| Last update date |
Aug 07, 2019 |
| Contact name |
Jennifer Tsoi |
| E-mail(s) |
JTsoi@mednet.ucla.edu
|
| Organization name |
UCLA
|
| Department |
University of California, Los Angeles
|
| Lab |
Thomas Graeber
|
| Street address |
570 Westwood Blvd, CNSI 4338
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE118748 |
Improved expansion, functionality and tumor targeting of adoptively transferred T cells with kinetically engineered IL-2 |
|
| Relations |
| BioSample |
SAMN09862042 |
| SRA |
SRX4575820 |
