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Sample GSM335222 Query DataSets for GSM335222
Status Public on Jun 01, 2009
Title EL4_CHX_PMAIoM_rep2
Sample type RNA
Source name 4h PMA/IoM Stimulated, CHX treated EL4 cells
Organism Mus musculus
Characteristics Treatment: Cycloheximide
Treatment: PMA and Ionomycin
cell line: EL4
Treatment protocol EL4 cells were pre-treated with either 10microg/mL cycloheximide (CHX) in DMSO or DMSO for 30 min and stimulated with PMA (10 ng/ml phorbol myristate acetate) and Ionomcyin (1 microM) for 0h or 4h
Growth protocol EL-4 were cultured in RPMI 1640 medium with 10 mM HEPES, 10% fetal calf serum (FCS; CSL, Australia), 120 microg/ml penicillin, and 16 microg/ml gentamycin.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI-reagent as per manufacturers instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
Hybridization protocol As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
Scan protocol Arrays were scanned on an Affymetrix Scanner 3000 7G
Description 4h Stimulated, CHX treated EL4 cells
Data processing Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
Submission date Oct 20, 2008
Last update date Oct 21, 2008
Contact name Kristine Hardy
Organization name University of Canberra
Lab Cytokine Gene Expression
Street address University of Canberra
City Bruce
State/province ACT
ZIP/Postal code 0200
Country Australia
Platform ID GPL6246
Series (2)
GSE13278 Expression analysis to identify inducible genes in T cells
GSE13279 Defining the chromatin signature of inducible genes in T cells

Data table header descriptions

Data table
10338068 5.697368145
10338069 3.811011791
10338070 3.998887539
10338071 3.830162525
10338072 4.089772224
10338073 5.578808784
10338074 10.36930943
10338075 6.231970787
10338076 7.663533211
10338077 10.89947701
10338078 5.455177784
10338079 4.689615726
10338080 5.195884228
10338081 6.305584431
10338082 4.869990349
10338083 3.765040874
10338084 5.861947536
10338085 9.334539413
10338086 4.39243412
10338087 5.632268429

Total number of rows: 35399

Table truncated, full table size 722 Kbytes.

Supplementary file Size Download File type/resource
GSM335222.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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