|
| Status |
Public on Jun 01, 2009 |
| Title |
EL4_CHX_PMAIoM_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
4h PMA/IoM Stimulated, CHX treated EL4 cells
|
| Organism |
Mus musculus |
| Characteristics |
Treatment: Cycloheximide
Treatment: PMA and Ionomycin
cell line: EL4
|
| Treatment protocol |
EL4 cells were pre-treated with either 10microg/mL cycloheximide (CHX) in DMSO or DMSO for 30 min and stimulated with PMA (10 ng/ml phorbol myristate acetate) and Ionomcyin (1 microM) for 0h or 4h
|
| Growth protocol |
EL-4 were cultured in RPMI 1640 medium with 10 mM HEPES, 10% fetal calf serum (FCS; CSL, Australia), 120 microg/ml penicillin, and 16 microg/ml gentamycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI-reagent as per manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
|
| |
|
| Hybridization protocol |
As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
|
| Scan protocol |
Arrays were scanned on an Affymetrix Scanner 3000 7G
|
| Description |
4h Stimulated, CHX treated EL4 cells
|
| Data processing |
Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
|
| |
|
| Submission date |
Oct 20, 2008 |
| Last update date |
Oct 21, 2008 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE13278 |
Expression analysis to identify inducible genes in T cells |
| GSE13279 |
Defining the chromatin signature of inducible genes in T cells |
|
