|
Status |
Public on Oct 16, 2009 |
Title |
6 h BH+LPS, biological rep2 |
Sample type |
RNA |
|
|
Source name |
6 h BH+LPS
|
Organism |
Mus musculus |
Characteristics |
RAW 264.7 cell line
|
Treatment protocol |
Samples were treated with 0.1 mg/mL Beta-Hematin. Immediately following treatment, lipopolysaccharide (LPS) was added at a final concentration of 1 μg/mL
|
Growth protocol |
Cells were cultured under standard incubation conditions (37 ºC, 5% CO2) and grown in RPMI supplemented with 5% FBS and 1μg/mL P/S
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were harvested at 6 h using Versagene RNA purification and DNase treatment kits. Six wells of a 6-well plate were pooled for each biological replicate.
|
Label |
biotin
|
Label protocol |
10ug of cRNA is reverse transcribed to make ss cDNA of the correct sense to hybridize to the probes on the array. A total of 5.5ug of this product is fragmented, and then labeled with Terminal Deoxy Transferase with Biotin
|
|
|
Hybridization protocol |
Arrays were hybridized as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
|
Scan protocol |
GeneChips were scanned using the The GeneChip Scanner as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
|
Description |
977DWW27
|
Data processing |
Signal intensities across microarrays were RMA normalized
|
|
|
Submission date |
Oct 20, 2008 |
Last update date |
Oct 22, 2008 |
Contact name |
David W Wright |
E-mail(s) |
David.Wright@vanderbilt.edu
|
Phone |
615-322-2636
|
Fax |
615-343-1234
|
Organization name |
Vanderbilt University
|
Department |
Chemistry
|
Street address |
SC Stevenson Center 7, Station B 351822
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37235-1822 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE13281 |
Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells |
|