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Status |
Public on Aug 10, 2010 |
Title |
gd T cells from calf 301 stimulated with vehicle only control. |
Sample type |
RNA |
|
|
Source name |
Bovine gamma delta T cells
|
Organism |
Bos taurus |
Characteristics |
gamma delta T cells from bovine calves
|
Treatment protocol |
Sorted cells were rested overnight then stimulated for 4 hours with either PBS or APP.
|
Growth protocol |
Peripheral blood was collected from 1 to 6 month old calves and gamma delta T cells were sorted to >97% purity.
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Extracted molecule |
total RNA |
Extraction protocol |
Rneasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using an Affymetrix 7G scanner
|
Description |
Gene expression data stimulated bovine gamma delta T cells 301_PBS
|
Data processing |
The data were imported into Genespring using RMA preprocessing for analysis.
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Submission date |
Oct 23, 2008 |
Last update date |
May 03, 2010 |
Contact name |
Jodi Fern Hedges |
E-mail(s) |
jodi.hedges@gmail.com
|
Phone |
406-994-6384
|
Organization name |
Montana State University
|
Department |
Veterinary Molecular Biology
|
Lab |
Jutila
|
Street address |
960 Technology Blvd.
|
City |
Bozeman |
State/province |
MT |
ZIP/Postal code |
59718 |
Country |
USA |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE13321 |
Changes in gene expression induced by apple polyphenol (APP) |
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