 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 01, 2018 |
| Title |
WT_1 |
| Sample type |
SRA |
| |
|
| Source name |
pro-B cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: pro-B cell
strain: C57BL/6
age: 6-7 weeks
hsc markers: B220+, CD19+, IgM-, IgD-, ckit+, CD25-
genotype/variation: wild type
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Pro-B cells were sorted directly into RLT (Qiagen) lysis buffer and RNA extracted with micro Rneasy columns. RNA quality and quantity was determined by a Bioanalyzer and normalized for library construction.
Libraries were prepared with the SMARTER method for low-input RNA using standard Illumni procedures.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and mapped to the mm9 genome with bowtie.
FPKM values and differential expression was determined using cuffdiff.
Genome_build: mm9
|
| |
|
| Submission date |
Sep 04, 2018 |
| Last update date |
Nov 01, 2018 |
| Contact name |
David Cantor |
| E-mail(s) |
David.Cantor@med.nyu.edu
|
| Organization name |
NYU School of Medicine
|
| Lab |
Gregory David Lab
|
| Street address |
550 First Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE119422 |
Identification of Bmi1 regulated genes in pro-B cells |
|
| Relations |
| BioSample |
SAMN09951205 |
| SRA |
SRX4637665 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
