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Status |
Public on Nov 01, 2018 |
Title |
WT_1 |
Sample type |
SRA |
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Source name |
pro-B cell
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Organism |
Mus musculus |
Characteristics |
cell type: pro-B cell strain: C57BL/6 age: 6-7 weeks hsc markers: B220+, CD19+, IgM-, IgD-, ckit+, CD25- genotype/variation: wild type
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Extracted molecule |
polyA RNA |
Extraction protocol |
Pro-B cells were sorted directly into RLT (Qiagen) lysis buffer and RNA extracted with micro Rneasy columns. RNA quality and quantity was determined by a Bioanalyzer and normalized for library construction. Libraries were prepared with the SMARTER method for low-input RNA using standard Illumni procedures.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
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Data processing |
Sequenced reads were trimmed for adaptor sequence and mapped to the mm9 genome with bowtie. FPKM values and differential expression was determined using cuffdiff. Genome_build: mm9
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Submission date |
Sep 04, 2018 |
Last update date |
Nov 01, 2018 |
Contact name |
David Cantor |
E-mail(s) |
David.Cantor@med.nyu.edu
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Organization name |
NYU School of Medicine
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Lab |
Gregory David Lab
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Street address |
550 First Avenue
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City |
New York |
State/province |
New York |
ZIP/Postal code |
10016 |
Country |
USA |
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Platform ID |
GPL17021 |
Series (1) |
GSE119422 |
Identification of Bmi1 regulated genes in pro-B cells |
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Relations |
BioSample |
SAMN09951205 |
SRA |
SRX4637665 |
Supplementary data files not provided |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data are available on Series record |
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