|
| Status |
Public on Apr 08, 2019 |
| Title |
03_brain_d0ZT8_1 |
| Sample type |
RNA |
| |
|
| Source name |
brain (ventral part of telenchephalon, hypothalamus and pituitary), SD (16 h before the transition to LD), replicate 1
|
| Organism |
Oryzias latipes |
| Characteristics |
tissue: brain (ventral part of telenchephalon, hypothalamus and pituitary)
gender: female
|
| Treatment protocol |
Medaka kept under SD conditions were transferred to LD conditions. Ventral part of telenchephalon, hypothalamus and pituitary were collected from six fish every 4 h for three days during this transition
|
| Growth protocol |
Medaka fish (Oryzias latipes) were obtained from a local dealer and kept outdoor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from three fish (n=2) to duplicate our observations using RNeasy tissue kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis and cRNA labeling reactions were performed with the Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Labeled cRNA was purified with RNeasy mini spin columns (QIAGEN) and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies).
|
| Scan protocol |
After washing with Gene Expression Wash Buffer (Agilent Technologies), the slide glass was scanned on a Microarray Scanner (Agilent Technologies).
|
| Description |
Gene expression under 16 h before the transition to LD.
|
| Data processing |
Microarray data were analyzed using the GeneSpring software (Agilent Technologies).
|
| |
|
| Submission date |
Sep 12, 2018 |
| Last update date |
Apr 09, 2019 |
| Contact name |
Takashi Yoshimura |
| E-mail(s) |
takashiy@agr.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Street address |
Furo-cho, Chikusa-ku
|
| City |
Nagoya |
| ZIP/Postal code |
4648601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23001 |
| Series (2) |
| GSE119837 |
Seasonal changes of gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) in Medaka fish (Oryzias latipes) |
| GSE119905 |
Seasonal regulation of the lncRNA LDAIR modulates self-protective behaviours during the breeding season |
|
