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Status |
Public on Apr 08, 2019 |
Title |
13_brain_d1ZT4_1 |
Sample type |
RNA |
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|
Source name |
brain (ventral part of telenchephalon, hypothalamus and pituitary), LD (4 h after the transition to LD), replicate 1
|
Organism |
Oryzias latipes |
Characteristics |
tissue: brain (ventral part of telenchephalon, hypothalamus and pituitary) gender: female
|
Treatment protocol |
Medaka kept under SD conditions were transferred to LD conditions. Ventral part of telenchephalon, hypothalamus and pituitary were collected from six fish every 4 h for three days during this transition
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Growth protocol |
Medaka fish (Oryzias latipes) were obtained from a local dealer and kept outdoor.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from three fish (n=2) to duplicate our observations using RNeasy tissue kit (QIAGEN).
|
Label |
Cy3
|
Label protocol |
cDNA synthesis and cRNA labeling reactions were performed with the Low Input Quick Amp Labeling Kit (Agilent Technologies).
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|
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Hybridization protocol |
Labeled cRNA was purified with RNeasy mini spin columns (QIAGEN) and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies).
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Scan protocol |
After washing with Gene Expression Wash Buffer (Agilent Technologies), the slide glass was scanned on a Microarray Scanner (Agilent Technologies).
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Description |
Gene expression under 4 h after the transition to LD.
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Data processing |
Microarray data were analyzed using the GeneSpring software (Agilent Technologies).
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Submission date |
Sep 12, 2018 |
Last update date |
Apr 09, 2019 |
Contact name |
Takashi Yoshimura |
E-mail(s) |
takashiy@agr.nagoya-u.ac.jp
|
Organization name |
Nagoya University
|
Street address |
Furo-cho, Chikusa-ku
|
City |
Nagoya |
ZIP/Postal code |
4648601 |
Country |
Japan |
|
|
Platform ID |
GPL23001 |
Series (2) |
GSE119837 |
Seasonal changes of gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) in Medaka fish (Oryzias latipes) |
GSE119905 |
Seasonal regulation of the lncRNA LDAIR modulates self-protective behaviours during the breeding season |
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