|
Status |
Public on Jun 18, 2020 |
Title |
4T1 HIF1B-WT_RNAseq_control_1day-Normoxia_r3b |
Sample type |
SRA |
|
|
Source name |
Breast tumor
|
Organism |
Mus musculus |
Characteristics |
tissue: breast treatment: DMSO 72h, 21% O2 24h growth protocol: cultured at 37°C in Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS), 5ml of 100 U/ml Penicillin-Streptomycin (Pen Strep, Life Technologies) and 5ml of L-Glutamine 200mM.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
PolyA mRNA capture by using KAPA Stranded mRNA-Seq Kit (Illumina) Strand specific library preparation
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
4T1 HIF1B-WT DMSO 1day-Normoxia
|
Data processing |
FastQ files were mapped to the reference genome (human:build GRCh37/hg19; mouse:mm10) using tophat (version 2.0.10). Counts were called byHTSeq (version 0.5.3p3) Genome_build: hg19; mm10 Supplementary_files_format_and_content: Raw read files contain raw read counts for each sample
|
|
|
Submission date |
Sep 12, 2018 |
Last update date |
Jun 19, 2020 |
Contact name |
Jieyi Xiong |
Organization name |
VIB-KULeuven Center for Cancer Biology
|
Street address |
Herestraat 49, box 912
|
City |
Leuven |
ZIP/Postal code |
3000 |
Country |
Belgium |
|
|
Platform ID |
GPL21103 |
Series (2) |
GSE85353 |
DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumour immunotolerance [RNA] |
GSE85356 |
DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumour immunotolerance |
|
Relations |
BioSample |
SAMN10038297 |
SRA |
SRX4673520 |