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Status |
Public on Sep 12, 2019 |
Title |
RT-18 v1 |
Sample type |
RNA |
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Source name |
human skin
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Organism |
Homo sapiens |
Characteristics |
timepoint: Pre treatment: Placebo
|
Treatment protocol |
Each subject was randomized to either treatment or placebo and treated for 42 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
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Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
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Data processing |
Processed using affy package for R, using RMA normalization. Brainarray custom CDF for HGU133A2 (version 21.0.0, ENTREZG) was utilized for this data set. Http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/entrezg.asp
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|
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Submission date |
Sep 13, 2018 |
Last update date |
Sep 13, 2019 |
Contact name |
Julio C Mantero |
Organization name |
Boston University School of Medicine
|
Department |
Rheumatology
|
Street address |
72 E Concord St
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL25577 |
Series (1) |
GSE119939 |
IL-1 inhibition in systemic sclerosis skin. |
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