|
| Status |
Public on Sep 29, 2018 |
| Title |
White_Blood_Cell-val.S32 |
| Sample type |
genomic |
| |
|
| Source name |
Human_WBC (WhiteBloodCell)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: White blood cells
gender: Male
phenotype: Mental delay and/or physical disability
diagnosis: Normal
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from white blood cell from peripheral blood using the Chemagic DNA Blood Kit (200 µl). Extracted DNA quality and quantity were estimated using a Nanodrop Spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
DNA was processed as follows. Restriction digested, PCR amplified, fragmented, labeled and hybridized using agents (Affymetrix Hyb Buffer Part1-4) according to the manufacturer`s instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix Fluidics Stations, and scanned using the GeneChip Scanner 3000.
|
| Description |
val.S32
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Command Console (AGCC version 4.0.0.1567). Data processing and analysis (CEL file generation, smoothing and segmentation) were used Chromosome Analysis Suite (version 2.1.0.16).
|
| |
|
| Submission date |
Sep 28, 2018 |
| Last update date |
Sep 29, 2018 |
| Contact name |
Junnam Lee |
| E-mail(s) |
jnlee@greencross.com
|
| Organization name |
GreenCross genome
|
| Street address |
Green Cross Genome, Green Cross Laboratories-3rd floor . 107 Ihyeon-ro 30 beon-g
|
| City |
Yongin-si |
| ZIP/Postal code |
11111 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE120624 |
Affymetrix CytoScan 750K and HD data for developmental delay and/or physical disability phenotypes samples |
|
