|
| Status |
Public on Jan 02, 2019 |
| Title |
S5-NL-2 |
| Sample type |
RNA |
| |
|
| Source name |
human skin
|
| Organism |
Homo sapiens |
| Characteristics |
skin phenotype: Non Lesional
tissue: skin
disease state: discoid lupus erythematosus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Skin biopsies placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
|
| Data processing |
processed using affy package for R, using RMA normalization. Brainarray custom CDF for HGU133A2 (version 21.0.0, ENTREZG) was utilized for this data set. Http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/entrezg.asp
|
| |
|
| Submission date |
Oct 03, 2018 |
| Last update date |
Jan 02, 2019 |
| Contact name |
Julio C Mantero |
| Organization name |
Boston University School of Medicine
|
| Department |
Rheumatology
|
| Street address |
72 E Concord St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL25577 |
| Series (1) |
| GSE120809 |
Transcriptome of discoid lupus erythematosus (DLE) skin |
|
