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Sample GSM3427410 Query DataSets for GSM3427410
Status Public on Oct 12, 2019
Title Tbr2 conditional knockout _ embryonic cortex stage E13_replicate 2
Sample type RNA
 
Source name tissue: Brain cortex, cell type: basal Progenitors
Organism Mus musculus
Characteristics age: Embryonic day 13
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FACS isolated BPs by using mirVana kit (ambion) following manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared by using Illumina TotalPrep-96 RNA amplification kit from Ambion # 4393543
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Illumina BeadArray Reader used to capture images with default setting.
Description E13_Mutant_replicate 2
Data processing Illumina GenomeStudiov2011.1 were used to obtain raw signal intensities. Background substracted signal intensity for the detected probes (Detection p value<0.05) were used for analysis. Probes detected in one array and not in other were not considered during analysis. Further, outlier probes were removed and data were quantile normalized (Bolstad BM et al., 2003) along with our microarray gene expression data of E13 and E16 (GSE45450).
 
Submission date Oct 12, 2018
Last update date Oct 12, 2019
Contact name Matthias Groszer
Organization name INSERM U839
Department Developmental Neuroscience
Street address 17, rue du Fer à Moulin
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL6887
Series (1)
GSE121180 Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice
Relations
Reanalyzed by GSM3436922

Data table header descriptions
ID_REF
VALUE Normalized signal intensity in log2
MutE13_40.Detection Pval

Data table
ID_REF VALUE MutE13_40.Detection Pval
ILMN_1212602 null 0.0267094
ILMN_1212603 null 0.1463675
ILMN_1212605 7.98317 0
ILMN_1212607 null 0.3450855
ILMN_1212612 8.92039 0
ILMN_1212614 null 0.9967949
ILMN_1212619 null 0.5886752
ILMN_1212623 null 0.4722222
ILMN_1212625 9.746941 0
ILMN_1212626 null 0.4519231
ILMN_1212628 null 0.3151709
ILMN_1212632 4.926037 0.004273504
ILMN_1212633 5.385355 0
ILMN_1212636 11.513172 0
ILMN_1212637 8.244719 0
ILMN_1212638 13.542132 0
ILMN_1212639 null 0.3087607
ILMN_1212644 null 0.1730769
ILMN_1212645 null 0.3301282
ILMN_1212646 9.963594 0

Total number of rows: 45281

Table truncated, full table size 1177 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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