|
Status |
Public on Oct 12, 2019 |
Title |
Tbr2 conditional knockout _ embryonic cortex stage E13_replicate 3 |
Sample type |
RNA |
|
|
Source name |
tissue: Brain cortex, cell type: basal Progenitors
|
Organism |
Mus musculus |
Characteristics |
age: Embryonic day 13
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from FACS isolated BPs by using mirVana kit (ambion) following manufacturer's protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared by using Illumina TotalPrep-96 RNA amplification kit from Ambion # 4393543
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Illumina BeadArray Reader used to capture images with default setting.
|
Description |
E13_Mutant_replicate 3
|
Data processing |
Illumina GenomeStudiov2011.1 were used to obtain raw signal intensities. Background substracted signal intensity for the detected probes (Detection p value<0.05) were used for analysis. Probes detected in one array and not in other were not considered during analysis. Further, outlier probes were removed and data were quantile normalized (Bolstad BM et al., 2003) along with our microarray gene expression data of E13 and E16 (GSE45450).
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|
|
Submission date |
Oct 12, 2018 |
Last update date |
Oct 12, 2019 |
Contact name |
Matthias Groszer |
Organization name |
INSERM U839
|
Department |
Developmental Neuroscience
|
Street address |
17, rue du Fer à Moulin
|
City |
Paris |
ZIP/Postal code |
75005 |
Country |
France |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE121180 |
Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice |
|
Relations |
Reanalyzed by |
GSM3436923 |