|
| Status |
Public on Oct 17, 2019 |
| Title |
Panc-1 TGF-β 2 |
| Sample type |
RNA |
| |
|
| Source name |
Panc-1 TGF-β
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: pancreatic ductal adenocarcinoma
cell line: Panc-1
agent: TGF-β
|
| Treatment protocol |
Panc-1 cells were treated with 0 or 10 ng/ml of TGF-β for 72 hours.
|
| Growth protocol |
Panc-1 cells were obtained and cultured as recommended by the supplier.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNeasy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Total RNA was isolated from Panc-1 cells and analyzed miRNA expression by microarray.
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Oct 17, 2018 |
| Last update date |
Oct 19, 2019 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21263 |
| Series (1) |
| GSE121369 |
MiRNA expression profiling in pancreatic ductal adenocarcinoma (PDAC) cells treated with TGF-b |
|
