|
| Status |
Public on Nov 21, 2008 |
| Title |
Sham 2 |
| Sample type |
RNA |
| |
|
| Source name |
PBS treated
|
| Organism |
Mus musculus |
| Characteristics |
2h treatment of MLE-15 cells
|
| Treatment protocol |
MLE-15 cells were treated with sham (PBS), NTHi lysate (100 ug/ml) or EF2505-III (40 ug/ml) for 2 hours.
|
| Growth protocol |
MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
|
| Label |
Cy3
|
| Label protocol |
cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to BeadChips per Illumina instructions.
|
| Scan protocol |
Chips were scanned using level 3 signal using a BeadStation 500 scanner.
|
| Description |
Sham treated
|
| Data processing |
Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
|
| |
|
| Submission date |
Nov 20, 2008 |
| Last update date |
Nov 20, 2008 |
| Contact name |
Scott E Evans |
| E-mail(s) |
seevans@mdanderson.org
|
| Organization name |
University of Texas-M.D. Anderson Cancer Center
|
| Department |
Pulmonary Medicine - Box 403
|
| Street address |
1400 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE13685 |
Gene expression in respiratory epithelial cells treated to induce StIR |
|
