|
| Status |
Public on Mar 05, 2019 |
| Title |
WT L4 A: Lactation day 4, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary Epithelial Cells, depleted of mammary adipocytes, at lactation day 4
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
Sex: female
tissue: Mammary Epithelial Cells depleted of mammary adipocytes
parity: 1st
stage of mammary development: Secretory Activation
|
| Treatment protocol |
Mammary Epithelial Cells (MEC) at pregnant day 14 and lactation day 4 were depleted of the mammary adipocytes by rapid enzymatic tissue digestion (PMID: 20739508), washed with PBS, pelleted, and snap frozen for total RNA expraction.
|
| Growth protocol |
FVB mothers were staged for pregnancy by post coital plug and sacrificed at either day 14 of pregnancy or day 4 of lactation (day 1 is the first morning litters are born).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was cleaned up and DNA was digested on column according to the Qiagen Rneasy mini kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated sense-strand DNA targets from total RNA were generated according to the Affymetrix WT PLUS Reagent Kit for GeneChip® Whole Transcript (WT) Expression Arrays
|
| |
|
| Hybridization protocol |
Array Strips Hybridization for the GeneAtlas® Instrument was carried out according to the Affymetrix protocol hybridizing array strips on the GeneAtlas® System.
|
| Scan protocol |
Array strips were scanned using the GeneAtlas™ Imaging Station according to the Affymetrix protocol.
|
| Description |
Gene expression data from MECs following secretory activation.
|
| Data processing |
Raw data were GC-RMA (robust multiarray averaging) normalized, log2 transformed, and statistics performed using the Partek Genomic Suite (Partek, St. Louis, MO).
|
| |
|
| Submission date |
Oct 25, 2018 |
| Last update date |
May 31, 2022 |
| Contact name |
Michael C. Rudolph |
| E-mail(s) |
Michael-rudolph@ouhsc.edu
|
| Organization name |
OUHSC
|
| Department |
Physiology
|
| Lab |
Rudolph Lab
|
| Street address |
975 NE 10th St. BRC North rm 364
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE121819 |
Liver X Receptor α Activation Enhances Cholesterol Secretion in Lactating Mammary Epithelium |
|
