|
| Status |
Public on Dec 08, 2011 |
| Title |
IM71_case |
| Sample type |
RNA |
| |
|
| Source name |
Intima media; type 2 diabetic patient
|
| Organism |
Homo sapiens |
| Characteristics |
Intima media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
|
| Label |
Biotin
|
| Label protocol |
One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Intima media
|
| Data processing |
The R statistical software was applied for data analysis. VSN was used for global background correction and normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values.
|
| |
|
| Submission date |
Nov 28, 2008 |
| Last update date |
Dec 08, 2011 |
| Contact name |
Vibe Skov |
| E-mail(s) |
vihs@regionsjaelland.dk
|
| Phone |
+4526178735
|
| Organization name |
Roskilde Hospital
|
| Department |
Hematology
|
| Street address |
Koegevej 7-13
|
| City |
Roskilde |
| ZIP/Postal code |
4000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE13760 |
Gene expression profiling in arterial tissue from type 2 diabetic patients |
|
