|
| Status |
Public on Dec 05, 2008 |
| Title |
SK-ES-1 |
| Sample type |
protein |
| |
|
| Source name |
EWS
|
| Organism |
Homo sapiens |
| Characteristics |
EWS
|
| Growth protocol |
Cells from each cell line were grown on a 100mm dish to about 80% confluency.
|
| Extracted molecule |
protein |
| Extraction protocol |
Media were removed and ice-cold 1x cell lysis buffer were added (2ml per 100mm dish); lysates were collected by scrapping and then incubated on ice for 30min; after centrifuging at 13000rpm on a desktop centrifuge for 10min; supernatant was collected; the
|
| Label |
biotinylated phospho-tyrosine antibodies
|
| Label protocol |
According to manufacturer's protocol
|
| |
|
| Hybridization protocol |
antibody coupled beads were incubated with total protein lysates to capture targeted analytes; the beads were then labelled with biotinylated phospho-tyrosine antibodies and streptavidin, R-phycoerythrin conjugates
|
| Scan protocol |
The data were collected with a Luminex 100 instrument
|
| Description |
antibodies against the analytes were coupled to beads of corresponding color; total mouse, rat or rabbit IgG were used as negative controls; a phospho-tyrosine peptide was coupled to beads as a positive control
raw data: E3 of Output 050307-2.csv
|
| Data processing |
The background readings for each capture antibody were obtained using microspheres incubated with 1x cell lysis buffer (Cell Signaling Technology). Values were considered positive if they were 3-fold over the background and represented by log2-transformation of the folds over background. Negative values were threshold to -5.
|
| |
|
| Submission date |
Dec 03, 2008 |
| Last update date |
Dec 04, 2008 |
| Contact name |
Jinyan Du |
| E-mail(s) |
jinyandu@broad.mit.edu
|
| Phone |
617-324-4826
|
| Organization name |
The Broad Institute
|
| Department |
Cancer Program
|
| Lab |
4175-NN
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL7696 |
| Series (1) |
| GSE13808 |
Bead-based kinase phosphorylation profiling identifies SRC as a therapeutic target in glioblastoma |
|
