|
| Status |
Public on Jul 22, 2009 |
| Title |
WT-OVX-1 |
| Sample type |
other |
| |
|
| Source name |
uterus from ovariectomized wild type mouse
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: female
Tissue: the uterus
|
| Treatment protocol |
At 15 week old, female mice were ovariectomized, and after one week uteruses were dissected.
|
| Growth protocol |
16 weeks old
|
| Extracted molecule |
other |
| Extraction protocol |
To isolate miRNA fractions from total RNA, 100μｇ total RNA was fractionated and cleaned up with the flashPAGETM fractionator System and reagents (Ambion) per the manufacturer’s recommendation.
|
| Label |
Cy5
|
| Label protocol |
mirVanaTM miRNA Labeling Kit (Ambion) and amine-reactive dyes as recommended by the manufacturer.
|
| |
|
| Hybridization protocol |
A 3x miRNA Hybridization Buffer (Ambion) was added to the fluorescently labeled miRNAs and the solution was heated at 95ºC for 3 min. Slides were hybridized form 12 to 16 h at 42 ºC in sealed cassettes using a water bath. Following hybridization, the slides were washed and dried prior to a high-resolution scan.
|
| Scan protocol |
Scan on a GenePix 4000B (Axon Instruments, CA, USA). Raw data were normalized and analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA).
|
| Description |
Vehicle control of ovariectomized wild type mouse
|
| Data processing |
We used Array-Pro Analyzer Ver4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) to determine the signal intensity of each spot and its local background on microarrays. We calculated net intensity by subtracting the mean intensity of all pixels within the local background area from the mean intensity of all pixels within the spot areas.
|
| |
|
| Submission date |
Dec 08, 2008 |
| Last update date |
Jul 22, 2009 |
| Contact name |
kaoru yamagata |
| E-mail(s) |
ykaoru7@hotmail.com
|
| Organization name |
university of tokyo
|
| Street address |
yayoi 1-1-1
|
| City |
bukyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5106 |
| Series (2) |
| GSE13858 |
Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment |
| GSE14031 |
Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo |
|
