GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM348315 Query DataSets for GSM348315
Status Public on Jul 22, 2009
Title WT-OVX-1
Sample type other
Source name uterus from ovariectomized wild type mouse
Organism Mus musculus
Characteristics Strain: C57BL/6
Gender: female
Tissue: the uterus
Treatment protocol At 15 week old, female mice were ovariectomized, and after one week uteruses were dissected.
Growth protocol 16 weeks old
Extracted molecule other
Extraction protocol To isolate miRNA fractions from total RNA, 100μg total RNA was fractionated and cleaned up with the flashPAGETM fractionator System and reagents (Ambion) per the manufacturer’s recommendation.
Label Cy5
Label protocol mirVanaTM miRNA Labeling Kit (Ambion) and amine-reactive dyes as recommended by the manufacturer.
Hybridization protocol A 3x miRNA Hybridization Buffer (Ambion) was added to the fluorescently labeled miRNAs and the solution was heated at 95ºC for 3 min. Slides were hybridized form 12 to 16 h at 42 ºC in sealed cassettes using a water bath. Following hybridization, the slides were washed and dried prior to a high-resolution scan.
Scan protocol Scan on a GenePix 4000B (Axon Instruments, CA, USA). Raw data were normalized and analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA).
Description Vehicle control of ovariectomized wild type mouse
Data processing We used Array-Pro Analyzer Ver4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) to determine the signal intensity of each spot and its local background on microarrays. We calculated net intensity by subtracting the mean intensity of all pixels within the local background area from the mean intensity of all pixels within the spot areas.
Submission date Dec 08, 2008
Last update date Jul 22, 2009
Contact name kaoru yamagata
Organization name university of tokyo
Street address yayoi 1-1-1
City bukyo-ku
State/province Tokyo
ZIP/Postal code 113-0032
Country Japan
Platform ID GPL5106
Series (2)
GSE13858 Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment
GSE14031 Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

Data table header descriptions
VALUE Net Intensity after Normalization

Data table
BM10004 218.2471123
BM10017 149.3252073
BM10019 223.9297118
BM10022 175.5527598
BM10030 175.327065
BM10032 143.7364618
BM10034 148.6771723
BM10035 154.536305
BM10048 11626.32962
BM10049 158.0602763
BM10050 12166.99216
BM10053 147.6984153
BM10056 1429.561379
BM10057 277.0350863
BM10063 163.2557318
BM10064 244.3718728
BM10065 150.2682105
BM10067 313.5596958
BM10070 402.3986303
BM10071 149.8145858

Total number of rows: 662

Table truncated, full table size 12 Kbytes.

Supplementary file Size Download File type/resource
GSM348315.jpg.gz 1.6 Mb (ftp)(http) JPG
GSM348315.txt.gz 15.8 Kb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap