|
Status |
Public on Feb 27, 2019 |
Title |
D1_4 |
Sample type |
SRA |
|
|
Source name |
peripheral blood mononuclear cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood condition: subacute ischemic stroke
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. RNA libraries were prepared for sequencing using standard Illumina protocols after removing the ribosome RNA
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13 HISAT2.1.0 mapping to reference genome count counting Genome_build: GRCh38 Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
Nov 19, 2018 |
Last update date |
Feb 27, 2019 |
Contact name |
lili tian |
E-mail(s) |
tianlili01@hotmail.com
|
Organization name |
tianjin medical university general hospital
|
Street address |
NO.154 Anshan Road
|
City |
tianjin |
ZIP/Postal code |
300070 |
Country |
China |
|
|
Platform ID |
GPL20795 |
Series (1) |
GSE122709 |
LncRNA expression profiling of ischemic stroke patients |
|
Relations |
BioSample |
SAMN10441770 |
SRA |
SRX5027117 |