|
| Status |
Public on Feb 27, 2019 |
| Title |
NC_1 |
| Sample type |
SRA |
| |
|
| Source name |
peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
condition: healthy control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols after removing the ribosome RNA
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GRCh38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Nov 19, 2018 |
| Last update date |
Feb 27, 2019 |
| Contact name |
lili tian |
| E-mail(s) |
tianlili01@hotmail.com
|
| Organization name |
tianjin medical university general hospital
|
| Street address |
NO.154 Anshan Road
|
| City |
tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE122709 |
LncRNA expression profiling of ischemic stroke patients |
|
| Relations |
| BioSample |
SAMN10441758 |
| SRA |
SRX5027124 |
