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Sample GSM3494876 Query DataSets for GSM3494876
Status Public on May 14, 2019
Title YF_2
Sample type SRA
 
Source name Ophiocordyceps sinensis
Organism Ophiocordyceps sinensis
Characteristics strain: CGMCC 3.16320
Extracted molecule total RNA
Extraction protocol Ten individuals were pooled as one replicate and three replicates were collected for each sample. After collection, these samples were immediately frozen in liquid nitrogen and stored at _80°C refrigerator until RNA extraction.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description The samples of stroma length 1_4cm young fruitbody (YF)
Data processing Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit for Illumina®
The library quality was assessed on an Agilent Bioanalyzer 2100 system
The cDNA library was sequenced on an Illumina Hiseq 2000 platform at Novogene Bioinformatics Technology Co., Ltd
Clean data (clean reads) were obtained by removing reads containing an adapter, reads containing poly_Ns and low quality reads from raw data through in_house perl scripts, on which all following of the analyses were based.
Genome_build: PRJNA382001
Genome_build: http://www.plantkingdomgdb.com/Ophiocordyceps_sinensis/
Supplementary_files_format_and_content: Ophiocordyceps_sinensis_gene_fpkm excel include FPKM values for each Sample
 
Submission date Nov 28, 2018
Last update date May 14, 2019
Contact name CH Dong
E-mail(s) dongch@im.ac.cn
Phone +86-10-64806138
Organization name Chinese Academy of Sciences
Department Institute of Microbiology
Lab State Key Laboratory of Mycology
Street address NO.3 Park 1, Beichen West Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL25863
Series (1)
GSE123085 Ophiocordyceps sinensis raw sequence reads
Relations
BioSample SAMN10495292
SRA SRX5075534

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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