|
| Status |
Public on Apr 01, 2010 |
| Title |
mp_IAA_REP2 |
| Sample type |
RNA |
| |
|
| Source name |
9 day-old mp-B4149 seedlings, DEX/IAA-treated, replica 2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Total seedling
|
| Treatment protocol |
Seedlings were treated with 10 micromolar Dexamethasone during one hour in liquid MS medium, and during 1 more hour with 50 micromolar IAA.
|
| Growth protocol |
Seedlings were grown on half-strength Murashige-Skoog (MS) media containing 1% sucrose and 0.8% Daichin Agar in a 16 hour light / 8 hour dark cycle at 23 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purified total RNA was isolated using Qiagen RNEasy columns
|
| Label |
biotin
|
| Label protocol |
cRNA synthesis and labeling was done according to Schmid et al., Nature Genetics [2005] 37, 501-506
|
| |
|
| Hybridization protocol |
Hybridisation of 15ug cRNA was done overnight for 16 hours at 45ºC in a Hybridisation Oven 640 (Affymetrix). The protocol is conducted as described in the Genechip Expression Analysis Technical Manual, section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Target Hybridization) (P/N 701027, revision 5).
|
| Scan protocol |
Arrays were scanned on an Affymetrix 3000 7G scanner, as described n the Genechip Expression Analysis Technical Manual, section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Arrays: Washing, Staining and Scanning (P/N 701028, revision 5).
|
| Description |
mp_B
|
| Data processing |
Expression estimates were calculated using GCRMA
|
| |
|
| Submission date |
Dec 09, 2008 |
| Last update date |
Nov 21, 2009 |
| Contact name |
Dolf Weijers |
| E-mail(s) |
dolf.weijers@wur.nl
|
| Phone |
+31-317-482866
|
| Fax |
+31-317-484801
|
| URL |
http://www.bic.wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Laboratory of Biochemistry
|
| Street address |
Dreijenlaan 3
|
| City |
Wageningen |
| ZIP/Postal code |
6703 HA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE13881 |
Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein |
|
