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| Status |
Public on Dec 07, 2021 |
| Title |
BAT4 |
| Sample type |
SRA |
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| Source name |
Brown Adipose
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| Organism |
Mus musculus |
| Characteristics |
tissue: Brown Adipose
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| Extracted molecule |
total RNA |
| Extraction protocol |
Adipose tissues were collected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
RNA-seq_FPKM.xlsx
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| Data processing |
Reads were aligned to mm10, using Tophat (version tophat-2.0.9) and genes were quantified using Cufflinks (Version 2.1.1)
Genome_build: mm10
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| Submission date |
Dec 07, 2018 |
| Last update date |
Dec 07, 2021 |
| Contact name |
Lei Sun |
| E-mail(s) |
sun.lei@duke-nus.edu.sg
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| Organization name |
Duke-NUS
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| Street address |
8 College Road
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| City |
Singapore |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
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| Platform ID |
GPL13112 |
| Series (1) |
| GSE123511 |
Identification of a natural beige adipose depot in mice |
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| Relations |
| BioSample |
SAMN10534503 |
| SRA |
SRX5122766 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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