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Sample GSM350958 Query DataSets for GSM350958
Status Public on Aug 27, 2009
Title small airways, smoker 246
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 48
sex: F
ethnic group: black
smoking status: smoker, 26.5 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description S-246_sm
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 12, 2008
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (8)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE13933 Trachea Epithelium as a “ Canary” for Cigarette Smoking-induced Biologic Phenotype of Small Airway Epithelium
GSE17905 Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE MAS5-calculated signal intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 777.01 P 0.000389797
AFFX-BioB-M_at 1261.84 P 4.42873e-05
AFFX-BioB-3_at 701.045 P 5.16732e-05
AFFX-BioC-5_at 2332.59 P 0.000296708
AFFX-BioC-3_at 3177.93 P 4.42873e-05
AFFX-BioDn-5_at 5095.2 P 4.42873e-05
AFFX-BioDn-3_at 9920.25 P 7.00668e-05
AFFX-CreX-5_at 26815.4 P 5.16732e-05
AFFX-CreX-3_at 31546.8 P 4.42873e-05
AFFX-DapX-5_at 4.59713 A 0.834139
AFFX-DapX-M_at 36.1329 A 0.287743
AFFX-DapX-3_at 55.4839 A 0.60308
AFFX-LysX-5_at 4.53344 A 0.814869
AFFX-LysX-M_at 12.6857 A 0.617401
AFFX-LysX-3_at 60.8883 P 0.0309764
AFFX-PheX-5_at 13.6685 A 0.574038
AFFX-PheX-M_at 5.90559 A 0.971543
AFFX-PheX-3_at 28.3295 A 0.559354
AFFX-ThrX-5_at 7.97426 A 0.910522
AFFX-ThrX-M_at 9.01949 A 0.617401

Total number of rows: 54675

Table truncated, full table size 1637 Kbytes.




Supplementary file Size Download File type/resource
GSM350958.CEL.gz 8.2 Mb (ftp)(http) CEL
GSM350958.CHP.gz 13.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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