|
| Status |
Public on Dec 18, 2008 |
| Title |
FHC - control 22 |
| Sample type |
RNA |
| |
|
| Source name |
Control 22
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: male
Age: 22 years
Disease: none
Drugs: none
Tissue: blood
|
| Biomaterial provider |
Tadeja Režen
|
| Treatment protocol |
No treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood was collected in PaxGeneTM Blood RNA Tubes (PreAnalytiX GmbH, A Qiagen/BD Company, Switzerland). Total RNA from white blood cells was isolated using PaxGeneTM RNA Kit (PreAnalytiX GmbH, A Qiagen/BD Company, Switzerland) according to manufacturer’s protocol. DNAse I treatment was done on columns using RNase-Free DNase Set (Qiagen GmbH, Hilden, Germany) according to manufacturer’s recommendations.
|
| Label |
biotin
|
| Label protocol |
Sample was labeled using 1 µg of total RNA and GeneChip® Expression 3’-Amplification One-Cycle Target Labeling and Control Reagents Kit (Affymetrix, Santa Clara, CA, USA) according to manufacturer’s recommendations.
|
| |
|
| Hybridization protocol |
Sample was hybridized to GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA, USA), and washed using GeneChip® Fluidics Station 450 and user prepared solutions according to manufacturer’s recommendations.
|
| Scan protocol |
Slide was scanned using GeneChip® Scanner 3000 7G and images were analyzed using GCOS (GeneChip® Operating Software).
|
| Description |
A study in patients with familial hypercholesterolemia
|
| Data processing |
Data was normalized using Robust Multichip Average (RMA) as implemented in Expression Console and transformed to non-logarithmic data.
|
| |
|
| Submission date |
Dec 16, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Peter Juvan |
| Phone |
+386 1 543 7595
|
| Organization name |
Faculty of Medicine, University of Ljubljana
|
| Department |
Institute of Biochemistry
|
| Lab |
Center for Functional Genomics and Bio-Chips
|
| Street address |
Zaloska 4
|
| City |
Ljubljana |
| ZIP/Postal code |
SI-1000 |
| Country |
Slovenia |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13985 |
Atherosclerotic markers in human blood - a study in patients with familial hypercholesterolemia |
|
| Relations |
| Reanalyzed by |
GSE119087 |
