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Status |
Public on Dec 02, 2019 |
Title |
Drosophila simulans_zt15_18C |
Sample type |
SRA |
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Source name |
RNA from fly heads
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Organism |
Drosophila simulans |
Characteristics |
circadian timepoints: zt15 temperature: 18C tissue: fly heads
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Growth protocol |
Flies were grown at different temperatures and heads were collected at different temperatures.
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Extracted molecule |
polyA RNA |
Extraction protocol |
RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol. The precipitated RNAwas taken for library preparation. Libraries were constructed Stranded ligation-based, total-RNA libraries preparation was modified from (Engreitz et al., 2013) as described in Reddy et al 2018, Biorxiv.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Data processing |
quantification of tim isoforms looking manually the exon-exon juntion reads Genome_build: dm3, droAnanas2, droSim1, droViri3, droYak2 Supplementary_files_format_and_content: table of tim isoform analysis; transcript quantification
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Submission date |
Dec 19, 2018 |
Last update date |
Dec 02, 2019 |
Contact name |
Sebastian Kadener |
E-mail(s) |
skadener@gmail.com
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Organization name |
Brandeis University
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Street address |
415 South Street
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City |
Waltham |
State/province |
MA |
ZIP/Postal code |
9190401 |
Country |
USA |
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Platform ID |
GPL23865 |
Series (2) |
GSE124136 |
Differences in circadian rhythms in fly at different temperatures |
GSE124142 |
Total RNA from different species fly heads |
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Relations |
BioSample |
SAMN10616474 |
SRA |
SRX5167219 |
Supplementary data files not provided |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data are available on Series record |
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