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| Status |
Public on Dec 02, 2019 |
| Title |
Drosophila simulans_zt15_29C |
| Sample type |
SRA |
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| Source name |
RNA from fly heads
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| Organism |
Drosophila simulans |
| Characteristics |
circadian timepoints: zt15
temperature: 29C
tissue: fly heads
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| Growth protocol |
Flies were grown at different temperatures and heads were collected at different temperatures.
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol. The precipitated RNAwas taken for library preparation.
Libraries were constructed Stranded ligation-based, total-RNA libraries preparation was modified from (Engreitz et al., 2013) as described in Reddy et al 2018, Biorxiv.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
quantification of tim isoforms looking manually the exon-exon juntion reads
Genome_build: dm3, droAnanas2, droSim1, droViri3, droYak2
Supplementary_files_format_and_content: table of tim isoform analysis; transcript quantification
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| Submission date |
Dec 19, 2018 |
| Last update date |
Dec 02, 2019 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
skadener@gmail.com
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| Organization name |
Brandeis University
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| Street address |
415 South Street
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| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
9190401 |
| Country |
USA |
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| Platform ID |
GPL23865 |
| Series (2) |
| GSE124136 |
Differences in circadian rhythms in fly at different temperatures |
| GSE124142 |
Total RNA from different species fly heads |
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| Relations |
| BioSample |
SAMN10616455 |
| SRA |
SRX5167231 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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