|
| Status |
Public on Feb 23, 2009 |
| Title |
mesencephalon-diencephalon 12.5 day |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Tg mesencephalon-diencephalon 12.5
|
| Organism |
Mus musculus |
| Characteristics |
Tg mesencephalon-diencephalon embrionic 12.5 day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol
|
| Label |
Cy5
|
| Label protocol |
Standard protocol by Genisphere
|
| |
|
| Channel 2 |
| Source name |
WT mesencephalon-diencephalon 12.5
|
| Organism |
Mus musculus |
| Characteristics |
WT mesencephalon-diencephalon embrionic 12.5 day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol
|
| Label |
Cy3
|
| Label protocol |
Standard protocol by Genisphere
|
| |
|
| |
| Hybridization protocol |
ventana, 42C, 6 hours
|
| Scan protocol |
Scanarray Lite, Laser 100,100, PTM 80,80
|
| Description |
genepix
|
| Data processing |
Each spot was defined by automatic positioning of a grid of circles over the image. The average and median pixel intensity ratios calculated from both channels and the local background of each spot were determined. Local background corrected intensity ratios was determined for each spot. The background-corrected expression data were filtered for flagged spots and weak signal. Normalization was performed by the global Lowess method. Students t-test was applied to determine the p value.
|
| |
|
| Submission date |
Dec 20, 2008 |
| Last update date |
Feb 23, 2009 |
| Contact name |
Agnes Zvara |
| E-mail(s) |
zvara@brc.hu
|
| Organization name |
Biological Research Center
|
| Street address |
Temesvari krt 62
|
| City |
Szeged |
| ZIP/Postal code |
6726 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL7877 |
| Series (1) |
|
