|
| Status |
Public on Dec 24, 2019 |
| Title |
ASA01029 continuous 12 weeks |
| Sample type |
RNA |
| |
|
| Source name |
brushing of nasal epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
batch: batch 1
subject: ASA01029
dosing regimen: continuous
timepoint: 12 weeks
Sex: male
age: 65
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen miRNeasy Mini Kit following manufacturer’s instruction. Integrity of the RNA samples was assessed by Agilent BioAnalyzer, and purity of the RNA was confirmed using a NanoDrop spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
Gene expression from nasal epithelium of ASA01029 (continuous dosing regimen) at 12 weeks.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.48.0), using R version 3.2.3 and the Brainarray hugene10sthsentrezgcdf R package (version 20.0.0).
|
| |
|
| Submission date |
Jan 03, 2019 |
| Last update date |
Dec 24, 2019 |
| Contact name |
Adam C Gower |
| E-mail(s) |
agower@bu.edu
|
| Phone |
617-358-7138
|
| Organization name |
Boston University School of Medicine
|
| Department |
Department of Medicine
|
| Lab |
Division of Computational Biomedicine
|
| Street address |
72 East Concord Street, E632
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL23526 |
| Series (1) |
| GSE124637 |
The effect of intermittent versus continuous low dose aspirin on nasal epithelium gene expression in current smokers: a randomized, double-blinded clinical study |
|
