|
| Status |
Public on Jun 01, 2011 |
| Title |
Validation114 |
| Sample type |
RNA |
| |
|
| Source name |
Pretreatment sample_Validation set
|
| Organism |
Homo sapiens |
| Characteristics |
Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
|
| Description |
Validation set for the signature for the intrinsic resistance to CF
|
| Data processing |
The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
|
| |
|
| Submission date |
Dec 24, 2008 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Jeffrey E Green |
| E-mail(s) |
JEGreen@mail.nih.gov
|
| Phone |
3014355193
|
| Fax |
3014968709
|
| Organization name |
NCI
|
| Street address |
Rm 4054, 37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE14208 |
Clinical response of metastatic gastric cancer patients to cisplatin and fluorouracil (CF) combination chemotherapy |
| GSE14210 |
Expression data from human endoscopic biopsy samples |
|
