|
| Status |
Public on Oct 01, 2011 |
| Title |
Noto-GFP_positive_sort_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Noto-GFP positive cells sorted from the posterior of E8.5 mouse embryos
|
| Organism |
Mus musculus |
| Characteristics |
STRAIN: 129S3;ICR
GENDER: MIXED
|
| Treatment protocol |
The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
|
| Growth protocol |
Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
|
| Scan protocol |
Arrays were scanned using an Affymetrix GeneChip Scanner 3000
|
| Description |
replicate 2: posterior regions dissected and pooled from 39 embryos; ~4300 Noto-GFP positive cells sorted
|
| Data processing |
Data was analyzed using GCOS software v1.4
|
| |
|
| Submission date |
Dec 24, 2008 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Owen James Tamplin |
| Organization name |
University of Wisconsin-Madison
|
| Department |
Cell and Regenerative Biology
|
| Lab |
Tamplin
|
| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14211 |
Expression profiling of Noto-GFP+ notochord progenitor cells sorted from E8.5 mouse embryos |
|
