|
| Status |
Public on Sep 06, 2019 |
| Title |
PianpKO hypothalamus biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
PianpKO biological rep5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hypothalamus
genotype: KO
|
| Treatment protocol |
The whole brain from experimentally naive male mice was cut coronally and manually microdissected into the respective regions using brain punches. Allen mouse brain atlas (version 2, 2011, https://www.brain-map.org/) was used for identification of the different brain areas.
|
| Growth protocol |
Pianptm1a(KOMP)Wtsi mice were crossed with B6N.Cg-Tg(ACTFLPe)9205Dym/CjDswJ mice to generate Pianp floxed mice (FL). These mice were further bred with B6.C-Tg(CMV-cre)1Cgn/J mice to generate constitutive knockout mice, denoted as PianpKO (KO).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from dissected brain region tissue using the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data of Pianp-deficient murine hypothalamus.
Hypothalamus_KO_168
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 21 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Jan 08, 2019 |
| Last update date |
Sep 06, 2019 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23092 |
| Series (1) |
| GSE124791 |
Gene expression in murine amygdala, cortex, hippocampus and hypothalamus of Pianp-deficient mice |
|
