|
Status |
Public on Jan 25, 2019 |
Title |
F/F Tfr rep 2 |
Sample type |
SRA |
|
|
Source name |
F/F Tfr
|
Organism |
Mus musculus |
Characteristics |
tissue: lymph node cell type: Tfr genotype: F/F
|
Treatment protocol |
Ex Vivo Sorting
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Lymph nodes were mashed through 70micron filters and cells were sorted RNA libraries were constructed using previously published protocols using Illumina adaptors (Sage Nature Immunology 2016)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Basecalling was performed using Illumina Software Reads were aligned to the mouse genome mm10 using CLC Genomics software suite Reads per million were generated for each sample Genome_build: mm10 Supplementary_files_format_and_content: Tab-deliminated file
|
|
|
Submission date |
Jan 09, 2019 |
Last update date |
Jan 25, 2019 |
Contact name |
Peter Sage |
Organization name |
Brigham and Women's Hospital
|
Department |
Medicine/Renal
|
Lab |
Sage Lab
|
Street address |
221 Longwood Ave
|
City |
Boston |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE124881 |
FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program [Ezh2] |
GSE124884 |
FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program |
|
Relations |
BioSample |
SAMN10719887 |
SRA |
SRX5236296 |