|
| Status |
Public on Jan 01, 2024 |
| Title |
MEF Spry124 KO repA 120 min |
| Sample type |
RNA |
| |
|
| Source name |
Immortalized MEF
|
| Organism |
Mus musculus |
| Characteristics |
spry status: No Spry expression (Spry1,2,4 are excised)
culture genotype/variation: Serum Starved (DMEM + 0.2% BSA + 100 U/mL penicillin + 100ug/mL streptomycin)
fgf treatment time (min): 120
rep: A
genotype/variation: KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy Plus Mini Kit (Qiagen), according to manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Standard label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Spry124-/- MEFs
|
| Data processing |
The data were normalized using background subtraction and quantile normalization with Illumina Genome Studio (v 1.9.0)
|
| |
|
| Submission date |
Jan 10, 2019 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Neda Bagheri |
| E-mail(s) |
n-bagheri@northwestern.edu
|
| Phone |
847-491-2716
|
| Organization name |
Northwestern University
|
| Department |
Chemical and Biological Engineering
|
| Street address |
2145 Sheridan Road, Tech E154
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208-3109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE124923 |
Gene expression dynamics reveal Sprouty mediated regulation of Wnt pathway genes |
|
