|
Status |
Public on Jan 01, 2024 |
Title |
MEF Spry124 KO repA 120 min |
Sample type |
RNA |
|
|
Source name |
Immortalized MEF
|
Organism |
Mus musculus |
Characteristics |
spry status: No Spry expression (Spry1,2,4 are excised) culture genotype/variation: Serum Starved (DMEM + 0.2% BSA + 100 U/mL penicillin + 100ug/mL streptomycin) fgf treatment time (min): 120 rep: A genotype/variation: KO
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RNeasy Plus Mini Kit (Qiagen), according to manufacturer’s instructions
|
Label |
biotin
|
Label protocol |
Standard label protocol
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Spry124-/- MEFs
|
Data processing |
The data were normalized using background subtraction and quantile normalization with Illumina Genome Studio (v 1.9.0)
|
|
|
Submission date |
Jan 10, 2019 |
Last update date |
Jan 01, 2024 |
Contact name |
Neda Bagheri |
E-mail(s) |
n-bagheri@northwestern.edu
|
Phone |
847-491-2716
|
Organization name |
Northwestern University
|
Department |
Chemical and Biological Engineering
|
Street address |
2145 Sheridan Road, Tech E154
|
City |
Evanston |
State/province |
IL |
ZIP/Postal code |
60208-3109 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE124923 |
Gene expression dynamics reveal Sprouty mediated regulation of Wnt pathway genes |
|