|
| Status |
Public on May 15, 2019 |
| Title |
NORMAL PDAC1: Pooled histologically normal pancreatic - Pancreatic ductal adenocarcinoma 1 to 10 |
| Sample type |
RNA |
| |
|
| Source name |
histologically normal pancreatic
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: histologically normal pancreatic
gender: Pooled 1 to 10
age: Pooled 1 to 10
tumor grade: histologically normal pancreatic
tumor size: Not Applicable
nodal status: Not Applicable
distant metastasis: Not Applicable
Stage: Not Applicable
|
| Treatment protocol |
Samples obtained from FFPE tissue blocks were needle microdissected using the stereomicroscope Leica EZ4 (Leica Microsystems, Wetzlar, Germany) before RNA extraction, in order to isolate the target tumor or normal cell populations.
|
| Growth protocol |
NOT APPLICABLE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation (Thermo Fisher Scientific, Waltham, MA, USA) according to Goswami RS, Atenafu EG, Xuan Y, Waldron L, Reis PP, Sun T, et al. MicroRNA signature obtained from the comparison of aggressive with indolent non-Hodgkin lymphomas: potential prognostic value in mantle-cell lymphoma. J Clin Oncol [Internet]. 2013;31(23):2903–11.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 130 ng total RNA (FlashTag ™ Biotin HSR RNA Labeling Kit - Thermo Fisher Scientific, Waltham, MA, USA).
|
| |
|
| Hybridization protocol |
Following labeling, the biotin -labeled sample were hybridized for 16 hr at 48C on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Genechips Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) Software.
|
| |
|
| Submission date |
Jan 16, 2019 |
| Last update date |
May 15, 2019 |
| Contact name |
Tainara Francini Felix |
| E-mail(s) |
felix.tainara@gmail.com
|
| Phone |
14996257103
|
| Organization name |
UNESP
|
| Department |
Surgery and Orthopedics
|
| Street address |
Av. Prof. Mário Rubens Guimarães Montenegro, s/n, s/n
|
| City |
Botucatu |
| State/province |
SP |
| ZIP/Postal code |
18618-687 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE125179 |
microRNA modulated networks of adaptive and innate immune response in pancreatic ductal adenocarcinoma |
|
