|
| Status |
Public on Aug 31, 2009 |
| Title |
HUVEC_4 |
| Sample type |
RNA |
| |
|
| Source name |
Human Umbilical Vein Endothelial Cells HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
HUVEC cells from Clonetics Biowhittaker (Walkersville, MD)
|
| Growth protocol |
HUVEC cells were cultured in EGM-2MV media (Clonetics Biowhittaker).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen ECs with TRIzol reagent (Invitrogen, Carlsbad, CA), purified with the RNeasy total RNA Isolation Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms of total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridized for 16 hr and 30 minutes at 45°C on GeneChip HG-U133A Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
HG-U133A arrays were scanned using the Agilent GeneArray Scanner G2500A (Affymetrix).
|
| Description |
Gene expression profiling data of Umbilical Vein Endothelial Cells HUVEC_4
|
| Data processing |
The probe-level signals were converted to expression values using the Bioconductor function for Robust Multi-array Analysis (RMA), in which perfect match intensities are background adjusted, normalized by means of quantile-quantile normalization and log2-transformed.
|
| |
|
| Submission date |
Dec 30, 2008 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Luca Agnelli |
| E-mail(s) |
luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
|
| Phone |
+390223903581
|
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Department |
Department of Advanced Diagnostics
|
| Street address |
Venezian 1
|
| City |
MILAN |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE14230 |
Gene expression profiling of bone marrow endothelial cells in patients with multiple myeloma |
|
| Relations |
| Reanalyzed by |
GSE86363 |
