|
| Status |
Public on Jul 01, 2020 |
| Title |
Placental labyrinthine zone_control [MeoxxJJ E19 43-9_2] |
| Sample type |
RNA |
| |
|
| Source name |
Placental labyrinthine zone
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: Placental labyrinthine zone
developmental stage: E19
Sex: male
genotype: Control (Igf2+/flox)
|
| Treatment protocol |
Conceptuses were isolated from the mother's uterus at the embryonic day 19 (E19). Labyrinthine zone were micro-dissected under microscope from each placenta and flash-frozen in liquid N2 and stored at -80C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Midi Kits (Qiagen – 75144) and quantity and quality were verified using RNA 6000 Nano Kit (Agilent – 5067-1511) and an Agilent 2100 Bioanalyzer. Only RNA samples with RNA integrity numbers (RIN) >9.0 were used.
|
| Label |
biotin
|
| Label protocol |
The total RNA was subjected to reverse transcription to cDNA using random hexamers tagged with T7 promoter and this was subsequently used as a template for cRNA amplification. The cRNA was then subjected to another round of reverse transcription followed by biotin labeling as described in the Affymetrix (Santa Clara, CA, USA) GeneChip whole transcript sense target labeling assay manual and labeled using the Affymetrix whole transcript kit according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
The biotinylated ssDNA was hybridized onto Affymetrix GeneChip MouseGene 1.0 ST arrays, which screen the expression of 28,853 genes using 770,317 probes.
|
| Scan protocol |
Signals were detected using an Affymetrix GeneChip Scanner 3000 7G and analyzed using Partek Genomics Suite 6.4 software (St. Louis, MO, USA).
|
| Description |
RNA from E19 Mus musculus Placental labyrinthine zone - Control (Igf2+/flox)
|
| Data processing |
Array profiling was performed using the Mouse Gene 1.0 ST Array (Affymetrix) and the analysis of the data was performed using GeneSpring GX 12.1 (Agilent, Santa Clara, CA, USA), with two algorithms: RMA (Robust Multiarray Average) and PLIER (Probe Logarithmic Intensity Error).
|
| |
|
| Submission date |
Jan 17, 2019 |
| Last update date |
Jul 02, 2020 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE125278 |
Fetus-derived IGF2 matches placental development to fetal demand [microarray] |
| GSE125434 |
Fetus-derived IGF2 matches placental development to fetal demand |
|
