|
| Status |
Public on Jan 19, 2019 |
| Title |
Young_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
young MII_oocyte
|
| Organism |
Homo sapiens |
| Characteristics |
age: young
maternal age: <30
developmental stage: : metaphase II
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.
Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
single cell RNA seq
|
| Data processing |
Reads of RNA-seq were mapped to the human genome (GRCh38) with STAR
Mapped reads were subsequently assembled into transcripts guided by the reference annotation (GENCODE gene models) with Stringtie
Gene differential expression analysis using DESeq2 based on the reads count file obtained by HTSeq
Genome_build: hg38
Supplementary_files_format_and_content: Tab-delimited text files include read counts for each gene, FPKM
|
| |
|
| Submission date |
Jan 18, 2019 |
| Last update date |
Jan 20, 2019 |
| Contact name |
Jing-Jing Zhang |
| E-mail(s) |
jjzhang@webmail.hzau.edu.cn
|
| Organization name |
Huazhong Agricultural University
|
| Department |
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction
|
| Lab |
Miaolab
|
| Street address |
1 Lion Rock Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE125300 |
TOP2B disturbed the quality of human oocytes with advanced maternal age |
|
| Relations |
| BioSample |
SAMN10769171 |
| SRA |
SRX5260728 |
