|
| Status |
Public on Jan 19, 2019 |
| Title |
LV, WT2 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
tissue: left ventricle
age: 13 week-old
genotype: wild type (WT)
|
| Treatment protocol |
Left ventricule tissue from 10–13-week-old wild type (WT) and MURC knockout (KO) male mouse hearts. After sacrificing the mice, the hearts were excised by cervical dislocation and stored at -80 °C. Total RNA was extracted from the tissue using TRIzol reagent according to the manufacturer’s instructions.
|
| Growth protocol |
10-13 week old C57BL/6 background male mice (wild-type and MURC knockout) were prepared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total
|
| |
|
| Hybridization protocol |
RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene exprssion data from WT adult mouse heart
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 18, 2019 |
| Last update date |
Jan 19, 2019 |
| Contact name |
Masahiro Nishi |
| E-mail(s) |
nishim@koto.kpu-m.ac.jp
|
| Phone |
818014714541
|
| Organization name |
Kyoto prefectural university of medicine
|
| Department |
Cardiology
|
| Street address |
Kajii-cho 465, Kawaramachi Hirokoji, Kamigyo
|
| City |
Kyoto |
| ZIP/Postal code |
605-0036 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE125308 |
Expression data from wild-type and MURC knockout male mouse hearts |
|
