|
| Status |
Public on Jun 02, 2009 |
| Title |
cycle 14 haploid embryos, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
haploid embryos, 15min after the nuclear division of cycle 13
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Age: cycle 14
Genotype: maternal haploid;histone H2-GFP parents
|
| Treatment protocol |
Embryos were identified under Epi fluorescent microscope, timed from telophase of the previous cycle to 15min and 40min later respectively, hand-selected and frozen by dry-ice cold heptane. Triplicates of about 50 embryos each with right age were collected for each time point.
|
| Growth protocol |
Both diploid and haploid flies were raised at 25C to lay embryos on apple juice agar plate for 2-3hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
Gene chips were scaned using Affymetrix GeneChip Scanner 3000.
|
| Description |
Expression data from precisely staged blastula haploid Drosophila embryos.
Biological replicate 2 of 3.
|
| Data processing |
The data were analyzed with Microarray Suite using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 05, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Xuemin Lu |
| E-mail(s) |
xueminlu@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular biology
|
| Lab |
Wieschaus
|
| Street address |
Washington Rd
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE14287 |
Expression data from precisely staged blastula wild-type and haploid Drosophila embryos |
|
| Relations |
| Reanalyzed by |
GSE119084 |
