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Sample GSM358500 Query DataSets for GSM358500
Status Public on Jan 08, 2010
Title T4644B1
Sample type RNA
 
Source name primary colorectal adenocarcinoma
Organism Homo sapiens
Characteristics Location: Left; DukesStage: C; Age_Diag: 75; Gender: F; DFS_Time: 24.13; DFS_Cens: 0; AdjXRT: N; AdjCTX: Y
Biomaterial provider H Lee Moffitt Cancer Center
Extracted molecule total RNA
Extraction protocol Surgically isolated colorectal cancers were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using Trizol reagent (Invitrogen).
Label biotin
Label protocol Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Location: Left; DukesStage: C; Age_Diag: 75; Gender: F; DFS_Time: 24.13; DFS_Cens: 0; AdjXRT: N; AdjCTX: Y
Data processing Use the simpleaffy package in R/Bioconductor to calculate MAS5.0 calls. These values were subsequently normalized using quantile normalization.
 
Submission date Jan 08, 2009
Last update date Aug 28, 2018
Contact name Oliver Sieber
Phone 61-3-9341-3155
Fax 61-3-9341-3104
URL http://www.ludwig.edu.au
Organization name Ludwig Institute for Cancer Research
Department Melbourne Tumour Biology Branch
Lab LCCI laboratory
Street address 6th floor, Centre for Medical Research, Royal Melbourne Hospital, Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL570
Series (1)
GSE14333 Expression data from 290 primary colorectal cancers
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE log-base-2 values of quantile normalized MAS5.0 calls

Data table
ID_REF VALUE
1007_s_at 10.24165317
1053_at 8.004200164
117_at 8.8351523
121_at 8.669779206
1255_g_at 5.42862773
1294_at 6.853510663
1316_at 5.801518835
1320_at 2.704610835
1405_i_at 6.18932082
1431_at 4.753142276
1438_at 6.972845731
1487_at 7.342824869
1494_f_at 5.750530698
1552256_a_at 8.245951174
1552257_a_at 8.855654635
1552258_at 5.072086265
1552261_at 4.592289773
1552263_at 7.606589074
1552264_a_at 7.954983465
1552266_at 5.673989184

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM358500.CEL.gz 8.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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