|
| Status |
Public on Jan 08, 2010 |
| Title |
01-TB-008-1B |
| Sample type |
RNA |
| |
|
| Source name |
primary colorectal adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
Location: Left; DukesStage: C; Age_Diag: 69; Gender: M; DFS_Time: 15.2; DFS_Cens: 0; AdjXRT: N; AdjCTX: Y
|
| Biomaterial provider |
Royal Melbourne Hospital
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Surgically isolated colorectal cancers were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using Trizol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Location: Left; DukesStage: C; Age_Diag: 69; Gender: M; DFS_Time: 15.2; DFS_Cens: 0; AdjXRT: N; AdjCTX: Y
|
| Data processing |
Use the simpleaffy package in R/Bioconductor to calculate MAS5.0 calls. These values were subsequently normalized using quantile normalization.
|
| |
|
| Submission date |
Jan 08, 2009 |
| Last update date |
Jan 13, 2009 |
| Contact name |
Oliver Sieber |
| Phone |
61-3-9341-3155
|
| Fax |
61-3-9341-3104
|
| URL |
http://www.ludwig.edu.au
|
| Organization name |
Ludwig Institute for Cancer Research
|
| Department |
Melbourne Tumour Biology Branch
|
| Lab |
LCCI laboratory
|
| Street address |
6th floor, Centre for Medical Research, Royal Melbourne Hospital, Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14333 |
Expression data from 290 primary colorectal cancers |
|
